Abstract

A. Manoj*, B. Vishnu Bhat, C. Venkatesh and Z. Bobby

ABSTRACT

This study was aimed to calibrate one division of the ocular meter by using stage meter with 4X, 10X and 40X objectives of microscope and also to find out actual size of comet tail in manual mode. In 4X, the 1000 micrometer ruler of stage meter coincide with 40 divisions ruler of the ocular meter in which 25µm length was one division of oculometer. The comet tail length in 4X objective lens was 25 micrometer. Similarly, for 10 X objective eyepiece lens, 1000 µm ruler of stage micrometer coincide with entire 10 divisions ruler of ocular meter for which 10 µm was one ocular division. The extension of comet tail in 10X objective was 50 µm. In 40X objective lens 100 µm of stage micrometer coincide with 40 divisions of ocular meter, with distance of 2.5 micron in one ocular division. The tail length in 40X objective was 125 µm.