Abstract

Mrs. N. Sandhyarani*, Dilshad Begum, B. Kavya, Sumaiya Begum, K. Anusha, D. Mayuri Ramulu

ABSTRACT

Ceftazidime and Avibactam are combination antibiotic used to treatment of serious infections caused by susceptible gram – negative bacteria, including many multidrug resistant strains. Avibactam protects ceftazidime from bacterial β- lactamase enzyme thereby extending its activity. The present study aimed to develop and validate a simple, rapid, accurate, and economical UV spectrophotometric method for the estimation of ceftazidime and Avibactam in bulk and pharmaceutical dosage form. Ceftazidime and avibactam were dissolved in phosphate buffer (PH 7.0) and suitably to obtain the required concentrations and maximum absorbance were shown at 257nm and 226nm respectively. The developed method obeyed Beer–Lambert’s law over a concentration range of 2–20 µg/mL. Method validation was performed according to pharmaceutical regulatory guidelines, evaluating parameters such as linearity, accuracy, precision, sensitivity, limit of detection (LOD), and limit of quantification (LOQ). The calibration curve showed good linearity with a regression coefficient (R²) of 0.987. Accuracy was assessed through recovery studies, with percentage recoveries ranging from 98.7% to 101%. Precision studies demonstrated satisfactory reproducibility, with %RSD values less than 2%. The method exhibited good sensitivity, with the limit detection was 6.2215µg/ml and 6.23771µg/ml respectively. The limit of qualification was found to be 18.853µg/ml and 18.902µg/ml. respectively. The results demonstrated satisfactory recovery, low relative stand deviation (%RSD), and good reproducibility, indicating the reliability of the method. No interference from formulation excipients was observed, confirming the specificity of method. Owing to its simplicity, accuracy, precision, cost effective and reliability, the proposed UV spectrophotometric method is suitable for quality control analysis of ceftazidime and avibactam in bulk drug and pharmaceutical dosage form.