Abstract

R. Revathi*, V. Ganesan, E. Mohammed Thowfic Raja, A. Chelladurai, M. Premkumar, K. Sudharsan

ABSTRACT

The aim to develop the new ultra violet spectroscopic method for estimating the content of cefixime trihydrate (CT) using hydrotrophy phenomenon is to reduce the cost, time and environmental friendly. The hydrotropic solubilizing agent is used to increase the drug's aqueous solubility and to avoid the usage of organic solvents. Approximately 1% sodium bicarbonate solution was used as solvent to dissolve the pure drug as well to make the suitable dilution of marketed formulations. Absorbance of CT was measured at 287 nm (λ max). Then linearity plot was made in the concentration range of 10-100 μg/mL with y = 0.0098x - 0.0157 as regression equation. In accordance with ICH guidelines, this method was validated with accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ). The percentage recovery of CT was found between 98.68% and 99.77%. The %RSD values for intermediate precision and repeatability were lower than 2% proved the method precision. The values of LOD and LOQ, which were observed as 3.133µg/mL and 9.494µg/mL, respectively, use the regression equation. The suggested procedure was employed to commercial formulation and showed an assay percentage ranging from 99 to 101 percent. Therefore, instead of using other sophisticated method for frequent analysis, this quick, affordable, and eco friendly method can be used as a substitute for analysis of CT content in pure drug and finished dosage forms.