Abstract

Praveen Kumar*, Shivendra Singh, Usha, Sameer Trivedi, Shailja Singh, Ranjan Singh Rana, Kavyanjali Sharma and Amrita Bhashkar

ABSTRACT

Objective: Detection of renal tuberculosis (RTB) by real-time polymerase chain reaction (PCR) in renal biopsy tissue. Methods: Selected for fifty renal tuberculosis patients and twenty five healthy controls were taken in the present study. The renal biopsy tissue of these patients were used for Mycobacterium tuberculosis DNA detection by real-time PCR, using 35 and 40 as cycle threshold (CT) cut-off values. The detection of Microbacteria tuberculosis is used by Real Time PCR Kit (Shanghai ZJ Bio-Tech Co., Ltd.). Early morning urine samples collected for M. tuberculosis culture. Results: Fifteen patients in renal tuberculosis of, urine culture was Microbacteria tuberculosis positive. overall sensitivity of renal tuberculosis RT-PCR for detected of Microbacteria tuberculosis (CT40) cut off value result 86% sensitivity, 76% specificity, 87.75.% positive prediction value (PPV), 73.03% negative prediction value (NPV) and (CT35) cut of value result 78% sensitivity, 84.% specificity, 90.69.% positive prediction value (PPV), 65.62% negative prediction value (NPV). Urine PCR for IS6110 in detected of Microbacteria tuberculosis AFB result 30% sensitivity, 93.% specificity, 88.23.% positive prediction value (PPV), and 39.65% negative prediction value (NPV). Conclusions: Detection of M. tuberculosis DNA in renal biopsy tissue by real-time PCR is highly sensitive. M. tuberculosis culture is still the gold standard for rapid and superior diagnosis of renal tuberculosis by real-time PCR.