Abstract

Dr. Loveena*, Deeksha Kumari, Dr. Sumit Srivastava

ABSTRACT

Ayurveda, as an ancient system of holistic medicine, heavily emphasizes preventative care and environmental hygiene, critical components underpinning Panchakarma therapies. This longitudinal experimental study evaluated the microbiological contamination and antibiotic susceptibility patterns in a Panchakarma theatre within an Ayurvedic hospital over a three-month period. Twelve high-contact and environmental surfaces, including patient bed surfaces, therapeutic instruments, and waiting areas, were sampled monthly and analyzed using conventional culture techniques and disk diffusion for antibiotic resistance. The most frequent bacterial isolates included Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa, predominantly from moist and high-touch areas such as sinks, floors beneath therapy tables, and door handles. Notably, some traditionally emphasized therapeutic implements remained uncontaminated, reflecting the efficacy of single-use or traditional cleaning protocols. Antibiotic susceptibility testing revealed significant resistance to beta-lactams and fluoroquinolones among Gram-negative isolates, with maintained sensitivity to carbapenems and aminoglycosides. Integration of microbiological surveillance with classical Ayurvedic cleansing rituals, including dhoopana? (medicated fumigation), can amplify the prevention of nosocomial infections in such settings. This study highlights the necessity and possibility of harmonizing Ayurvedic tradition with modern infection control and antimicrobial stewardship, ensuring both patient safety and fidelity to holistic principles. Adoption of evidence-based microbiological protocols alongside traditional hygiene measures will foster a renewed paradigm in Ayurveda clinics, supporting their safe and sustainable operation in contemporary healthcare landscapes.