World Journal of Pharmaceutical
and Medical Research

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
ISSN (O) : 2455-3301
ISSN (P) : 3051-2557
IMPACT FACTOR: 6.842

ICV : 78.6

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SJIF Impact Factor: 6.842

Abstract

SCREENING AND CHARACTERIZATION OF ANTIBACTERIAL AGENTS FROM BUTEA MONOSPERMA (LAM.) TAUB SEED EXTRACTS

Dr. Lalitha V. M.Sc. Ph.D, *Dr. Kiran B. BE.d. M.Sc. M.Phil. Ph.D

ABSTRACT

In vitro antibacterial activity of Butea monosperma (Lam.) Taub. seed extract was evaluated against five bacterial species. Among the tested organisms, Proteus vulgaris exhibited the highest inhibition at 100% concentration, followed by Erwinia amylovora, Pectobacterium, Staphylococcus aureus, and Pseudomonas syringae. The inhibition zones ranged from 20.0 mm to 31.0 mm for E. amylovora and Pectobacterium, while P. vulgaris and S. aureus showed 24.0 mm and 25.0 mm inhibition, respectively. The methanolic seed extract was further subjected to thin-layer chromatography (TLC) to separate its bioactive components, yielding three fractions: Fraction I, Fraction II, and Fraction III. Among these, Fraction II demonstrated notable antibacterial activity against all tested strains, with marked inhibition observed in E. coli, Pectobacterium, P. vulgaris, P. syringae, and S. aureus. E. amylovora exhibited the greatest sensitivity, showing inhibition zones ranging from 31.0 mm to 39.0 mm across different concentrations, followed by P. vulgaris and S. aureus, which showed inhibition between 23.0 mm and 34.0 mm.

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