Abstract

Rajnikant*, Dr. K . Sarvanan, Mr. Lokendra Kumar, Mr. Ajeet Singh

ABSTRACT

The formulation and evaluation of soft gelatin capsules encapsulated with protein-digesting enzymes derived from pineapple extract, specifically bromelain, aim to enhance digestive health and provide targeted enzymatic action. This study explores the preparation of soft gelatin capsules containing pineapple extract rich in bromelain, with an emphasis on evaluating their stability, enzymatic activity, and bioavailability. The formulation process involved the selection of appropriate excipients, optimization of the encapsulation technique, and the determination of ideal encapsulation parameters. Enzyme activity was assessed through in vitro assays, while the release profile of bromelain was studied under different pH conditions to simulate gastrointestinal transit. The bioactivity and stability of the enzyme were also monitored over time. The results demonstrated the successful encapsulation of active bromelain with preserved enzymatic activity, suggesting that these soft gelatin capsules could serve as an effective oral supplement for promoting protein digestion and enhancing gut health. Furthermore, the evaluation of the product’s quality attributes, such as uniformity, dissolution, and stability, affirmed the formulation’s potential as a novel delivery system for digestive enzyme supplementation.