Abstract

Jumael E. F. Ralaivaon-Dratsitonta*, Mirado F. Fanomezantsoa, Mamy Andrianarijaona, Jacquelin A. Razanakoto, Tartin Andrianjato, Stanislas F. Lahadison and Léa H. Rasoanaivo

ABSTRACT

Gnidia daphnifolia L.f is among the Gnidia species endemic to Madagascar. It is a medicinal plant traditionally used in a treatment of several diseases including diarrhea, dysentery, fatigue and yellow fever or hepatitis. The aim of this work is to carry out the antioxidant of different extract and analgesic activity of ethyl acetate extract of Gnidia daphnifolia L.f stems. Different extraction methods such as Soxhlet extraction, reflux extraction and cold maceration have been carried out to obtain extracts. Phytochemical screening of the hydroethanolic extract allowed the presence of polyphenols, triterpene and steroids. Hexane, ethyl acetate and methanolic extracts were showed an antioxidant property. Methanolic extract had a significant antioxidant activity between these three extracts. Analgesic properties of ethyl acetate extract were conducted on experimental animals. At 50 et 100 mg/kg body weight of ethyl acetate extract, the results are significant compared of negative control. This extract inhibited both the pain induced by the acetic acid injection and the immersion of the mice’s tail in thermostatic water. The high-dose of this extract produced is toxic for the mice. So, these pharmacological properties could be due the presence of these families chemical compounds showed in phytochemical screening.