Abstract

*Yash B. Gagare, Sagar S. Gawade, Pradip R. Mandhare, Yuvraj B. Guldagad and Manoj L. Chaudhari

ABSTRACT

A simple and unique reversed-section high-performance liquid chromatography Method became developed and proven for the simultaneous determination of Sitagliptin (sita) and Dapagliflozin (dapa) in bulk and pharmaceutical dosage form. Chromatography turned into achieved on zorobax eclipse plus phenyl hexyl c18 (250 mm × four.6 mm, 5 μ particle length) column containing cell segment of Buffer: methanol: acetonitrile inside the ratio of 40:35:25% v/v. (1ml of Triethylamine is brought and ph is adjusted to a few.7 with zero.1% orthophosphoric Acid) at a drift fee of 1. Zero ml/minute. The analyte become monitored the use of image Diode array detector (pda) at 267 nm. The retention time turned into discovered to be 2.38 min. and 3.88 Minutes for met Sitagliptin and Dapagliflozin Respectively. Validation parameters specificity, linearity, accuracy, precision and Robustness had been found to be suited over the concentration stages of 100 μg/ml & 10 μg/ml for Sitagliptin (sita) and dapagliflozin (dapa) respectively. The Technique advanced has been statistically established in step with ich guidelines. The consequences of analysis had been proven as in line with international conference on Harmonization (ich) recommendations. Consequently the optimized approach can be Effectively applied for the simultaneous willpower of Sitagliptin and dapagliflozin inside the recurring quality Manipulate evaluation. Significance of evolved method is that, it is able to be utilized for Habitual or unknown sample evaluation of assay of Sitagliptin and dapagliflozin in pharmaceutical dosage form. Evolved by using diverse pharmaceutical industries. The proposed method become Located to be fast, correct, specific, particular, strong, rugged and cost-efficient.