Abstract

*Dr. P. Senthil Kumar and Dr. Ramanathan Ravi

ABSTRACT

Introduction: The purpose of this In -Vitro study was. Aim: To compare the antimicrobial efficacy of two different root canal irrigants two different root canal irrigants at two different concentrations (Chlorhexidine- 0.2% and 2% and NaOCl- 0.5% and 2%) against E.Fecalis in primary tooth- In-Vitro study. Materials and Methods: Sixty extracted human mandibular second primary molars were divided into four equal groups. Group-A- chlorhexidine- 2%, Group-B- Chlorhexidine-0.2%, Group-C- NaOCl-2.5% and Group-D- NaOCl-0.5%. Colony forming units were counted in order to check for the antibacterial efficacy of these root canal irrigants, against E.faecalis. Results: Before treatment and after treatment of every single sample were compared and between the groups. Colony forming units were counted and tabulated. Chi-square test and SPSS software were used for the statistical analysis of the results. Paired T-test with SPSS 15.0 version for windows, Kruskal-Wallis, Non-Parametric test was selected, as the mean value is less than two times of standard deviation. Post Hoc Test with Turkey HSD was calculated at p<0.05 for multiple comparison between the groups. Chlorhexidine-2% is very good antimicrobial irrigating solution against E.Faecalis amongst the tested irrigants as the colony forming units were reduced to the maximum when compared with other groups. Conclusions: Treatment protocols using irrigation with either NaOCl or CHX succeeded in significantly reducing the number of bacterial taxa and their levels in infected root canals, with significant difference between their concentrations. Chlorhexidine-2% and Sodium hypochlorite-2.5% have a good potential to keep a low E.Faecalis colony forming units count compare to the Lower concentration. Clinical Significance: Feasibility of Root canal irrigants, antibacterial efficacy at lower concentration in primary tooth were tested. Also, antibacterial reduction at different concentration of sodium hypochlorite and chlorhexidine will guide us in selection, for safe and effective concentration.