A RESEARCH ARTICLE ON HPTLC FINGERPRINT PROFILE OF VARIOUS PLANT EXTRACTS VIZ., ADOXA MOSCHATELLINA, ALPINIA GALANGA AND LAURUS NOBILIS AND THEIR FORMULATION
Adiba Afreen*, Dr. Anil Kumar Middha and Dr. Syed Hafeez Ahmed
ABSTRACT
The aim of present study was to report the chromatographic analysis and stability of individual formulation viz., Adoxa moschatellina (AM), Alpinia galanga (AG), Laurus nobilis (LN) and their combination-Polyherbal formulation (PHF) by using High Performance Thin Layer Chromatography (HPTLC) on 0,30,60 and 90 days samples. Each formulation was extracted using chloroform. Fingerprinting of formulations was done by using Silica gel plates and benzene: chloroform: methanol (2:4:0.5) as mobile phase using standard procedures and was scanned under 366 nm. The results showed that the formulation was stable and also showed good correlation between suspensions and standard. The corresponding Rf (Retardation factor) values for places 1, 2, 3, 4, 5, 6, 7, and 8 were 0.816, 0.828, 0.432, 0.529, 0.656, 0.386, 0.921 and 0.946. As a result, the HPTLC pattern remained unchanged for every formulation.
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