COMPARISON OF L-DOPA CONTENT IN TWO VARIETIES OF BROAD BEANS (VICIA FABA) BY DIFFERENT EXTRACTION TECHNIQUES
Ragni N. Vora, Ambika N. Joshi* and Nitesh C. Joshi
ABSTRACT
In the present study, attempts are made to develop suitable method(s) for extraction of L-DOPA from the powdered seeds of 2 varieties of Vicia faba using different solvents and conditions. The seed powder of both plants was subjected to 6 different extraction methods, where Method 1 was performed with different solvent ratios. All the extracts were analyzed using RP-HPLC and was validated according to The International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use guidelines. The L-DOPA extraction was best with Acetonitrile and formic acid mixture in cold maceration technique and overall gives good extraction efficiency in both the plants giving 5.063% and 4.422% L-DOPA in Vicia faba var major and Vicia faba var. minor, respectively. The present investigation was done to study the extraction efficiency of various extraction methods so to compare L-DOPA content in seed extracts of 2 varieties of Vicia faba.
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