Abstract

*Prabakar R., Arunkumar. S. and Rajasekaran. R.

ABSTRACT

In the present study was carried out on phytochemical screening and antibacterial activity of Cardiospermum helicacabum L. leaves and stems extract against some clinical pathogens. Phytochemical compounds were carried out on the different extract the powdered specimens using standard procedures. Soxhlet apparatus were used for the extracting antibacterial active compounds from the plant leaves and stem powered. In this investigation tannin, flavonoids and terpenoids showed positive results in Cardiospermum helicacabum L. leaves and stem aqueous extract and ethanol extract showed positive results in terpenoids, saponins, steroids, and carbohydrates. The discs were prepared and immersed various solvents extract prepared discs. Obtained pathogenic bacteria pure cultures were inoculated nutrient broth respectively. Antimicrobial susceptibility test was determined by Bauer et al., 1966 method. Antimicrobial activity against were analyzed against Bacillus subtilis Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia Pseudomonas aeruginosa. Ethanol and methanol extract was most effective followed by other aqueous extract. Staphylococcus aureus was more sensitive for ethanol and methanol extract of leaves and stem of the tested plants. The crude extract as well as the isolated compounds found to be active in this study could be useful for the development of new antibacterial drugs.