World Journal of Pharmaceutical
and Medical Research

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
ISSN 2455-3301
IMPACT FACTOR: 6.842

ICV : 78.6

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SJIF Impact Factor: 6.842

Abstract

STABILITY-INDICATING HPTLC METHOD FOR DETERMINATION OF EPLERENONE

Dr. Mrinalini C. Damle* and Snehal M. Dumbre

ABSTRACT

A simple and economical thin-layer chromatography /densitometry method has been developed for determination of eplerenone. The method was validated as per International Conference on Harmonization (ICH) guidelines. Aluminium TLC plates precoated with silica gel 60F254 were used as the stationary phase and ethyl acetate: toluene 7:3 (v/v) as mobile phase. A compact band (Rf 0.52 ± 0.03) was obtained for Eplerenone. Densitometric analysis was performed in the absorbance mode at 238 nm. Linear regression analysis revealed a good linear relationship with (R2 = 0.990 ± 0.0004) between peak area and concentration in the range 1500 - 9000 ng/band. The mean value of the slope and intercept were 2.29 and 7126.9 respectively.The limits of detection and quantitation were 114.06 ng/band and 345.64 ng/band, respectively. Eplerenone was subjected to acid , alkali hydrolysis, oxidation, photochemical and thermal degradation.

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