Abstract

Praveen Kumar Dasari*, Kumar Raja Jayavarapu, Sk. Reena Tabassum and Guduru Sai Krishna

ABSTRACT

In the present research, a simple, isocratic, new rapid, economical and cost-effective reverse phase highperformance liquid chromatographic (RP-HPLC) method was developed and validated for the quantification of simultaneous estimation of Chloroquine and Primaquine in its bulk and pharmaceutical dosage forms. High performance liquid chromatography was one of the most sophisticated methods for the analysis of compounds. The mobile phase and pH 3.0 phosphate buffer were optimized with contains methanol, mixed with phosphate buffer in the ratio of 70:30 % v/v. Inertsil C18 column C18 (4.6 x 150mm, 5?m) porous silica particles were taken as stationary phase. The detection was determined by using UV spectrum at 260 nm. These solutions were chromatography at a constant flow rate of 0.8 ml/min, the linearity range of Chloroquine and Primaquine were measured, that is from 100-500 ?g/ml of Chloroquine and 1-5?g/ml of Primaquine and linear regression coefficient was not more than 0.999. The values of % RSD are less than 2% indicates accuracy and precision of the method. The percentage recovery changes from 98-102% of Chloroquine and Primaquine, LOD and LOQ were observed within range. The validated parameters met as per the guidelines of ICH and USP. It concludes that the validated method was simple, accurate, precise and linear. The established method was suitable application in bulk and pharmaceutical dosage forms with high degree of accuracy and precision.