Abstract

Hannah Elizabeth S.*, Channamma G. M., Rita Saritha D'Souza, Joel Durairaj G., D. Gnanasekaran and Shyam Kumar B.

ABSTRACT

Objective of the study is the evaluation of Diuretic activity of aqueous, ethanolic and ethyl acetate, unripe pulp extract of Cucumis sativus (Linn.) in validated experimental animal models. It is belonging to the family Cucurbitaceae. It is a non-toxic natural therapeutic agent. The phytochemical investigations of extracts of Cucumis sativus L unripe pulp was performed for the identification of alkaloids, proteins, saponins, tannins, glycosides carbohydrates and triterpenes etc. An ethanol, ethyl acetate, aqueous extracts of unripe pulp of Cucumis sativus L was assessed in male albino rats using in-vivo Lipschitz test model. The volume of urine, electrolytes concentration, specific gravity and pH were the parameters of the study. The preliminary phytochemical screening showed the presence of carbohydrates, proteins, alkaloids, triterpenoids, saponins, glycosides. The interpretation of the result was done after subjecting the data obtained from study to appropriate statistical analysis which included one-way ANOVA followed by Dunnett’s Multiple Comparison Test. The result obtained from in-vivo studies showed that the extracts have significant diuretic activity. The result indicated that aqueous and ethanol extracts at 200, 500 mg/kg body weight shows significant (p<0.05) and electrolyte excretion (p<0.001) when compared to control. The result of the study have shown diuretic activity with the extracts of Cucumis sativus (Linn.). Further, experimental and clinical studies are required to elucidate the chemical constituents of the extract and mechanism responsible for the diuretic activity.