Abstract

Amit S. Sontakke* and Kailash R. Biyani

ABSTRACT

The objective of this study was to evaluate the phytochemical profile, antioxidant activity and α- amylase inhibitory activity of an antidiabetic polyherbal formulation (PHF) that included Eugenia Jambolana L., Gymnema sylvestre Retz, Momordica charantia L, and Trigonella foenum-graecum L. Methods. Standard procedures were used to determine the total phenolic content, flavonoids, and flavonols in the formulation and to evaluate α- amylase inhibitory activity of the formulation. Total antioxidant activity, ferric reducing antioxidant power, and 2-diphenylpicrylhydrazyl (DPPH) assays were used to determine antioxidant potential. Results. The PHF contained 213.4 ± 0.72 mg gallic acid equivalent/g of total phenolic content. The PHF had 152.6 ± 0.45 mg quercetin equivalent/g of total flavonols and 126.2 ± 0.34 mg quercetin equivalent/g of total flavonoids. The reducing power of the PHF was 18.4 mg ascorbic acid equivalent/g. The total antioxidant capacity of 126.2 mg ascorbic acid equivalent/g was discovered. The PHF had a ferric reducing antioxidant power of PHP was 1024.4 (μM Fe (II)g). The formulation had a DPPH radical scavenging activity value was 1.8μg/mL for gallic acid and 2.6 μg/mL for the formulation. The IC50 value of α-amylase inhibition for water extract and hydroalcohol extract was found to be 8.425 and 10.242 mg/ml respectively Conclusions. The findings indicate that the polyherbal formulation has high antioxidant activity and could be a promising option for anti-diabetic research.