Abstract

Ashim M.K. and Menakshi Pachori*

ABSTRACT

Introduction- Identification and evaluation of antimutagenic compounds and their mechanisms of action are of great significance in protecting human health. However, the genotoxic potential of many rasayanas remains to be evaluated. Aims and objectives- The aim of this study is to verify the antimutagenic activity (i.e., effect on genotoxicity) of Brahmarasayana against some standard mutagens in human lymphocyte cultures. As it will be beneficial for further in vivo studies and clinical studies regarding the prevention and treatment of diseases like cancer. Material and methods- Antimutagenic study was done in human lymphocytes. CBMN assay was performed in all the cultures and the level of genetic damage was assessed. In this study the antimutagenic activity of test drug Brahmarasayana was assessed by counting the MN frequency in not less than 1000 binucleated cells. Observations and results- The experimental study was carried out in lymphocytes of a healthy individual. The blood samples collected was divided into 22 and separate lymphocytic cultures were prepared viz 1,2,3,4,5,…,22. In Culture 1, no extracts of study drug Brahmarasayana was added which is the positive control, and in Culture 22 500 μg/ml aqueous extract of Brahmarasayana was added and no bleomycin (a mutagenic substance) was added. While in Culture 2, 3, 4, 5,…,21 the aqueous extract of Brahmarasayana was added at a concentration of 50 μg/ml, 100 μg/ml, 150 μg/ml, 200 μg/ml, 250 μg/ml, 300 μg/ml, 350 μg/ml, 400 μg/ml, 450 μg/ml, 500 μg/ml, 550 μg/ml, 600 μg/ml, 650 μg/ml, 700 μg/ml, 750 μg/ml, 800 μg/ml, 850 μg/ml, 900 μg/ml, 950 μg/ml, and 1000 μg/ml respectively. Discussion- There are many Ayurvedic herbal products explained for natural rejuvenation. Among them, Brahmarasayana stands as the choicest natural remedy for chronic stress and tiredness and very useful to increase life span. Brahmarasayana should be consumed daily equal to one time food (Ahara thulya mathra). The dosage of avaleha kalpana is 1 pala (48g). The statistical analysis of the sample collected after testing showed that Brahmarasayana has antimutagenic effect. Conclusion- The study revealed that Brahmarasayana is effective in reducing the micronuclei frequency thereby helping in preventing the genetic damage.