Abstract

Suryam Gugulothu*, Manjula Jatoth, Rajitha E, Gayathri Paturi, Shravani G., Jatoth Thabitha, Pasala Shalini and Chandragiri Sridivya

ABSTRACT

The present study aims at formulating Nano sized green tea extract by the method of nanoprecipitation. The prepared nanoparticle was evaluated for particle size, zeta potential, electron Scanning Microscopy (SEM), percentage of encapsulation efficiency (EE) and in vitro drug release. To formulate Nano gel, the selected formulation of Green Tea Extract Nanoparticles has been integrated into a gelling agent. The Box-Behnken method has prepared a total of 17 formulations used to test the effect of three selected independent variables such as Carbapol 934 (X1), triethanolamine (X2), and stirring speed (X3). The dependent variables analysed were the Nanogel pH (Y1), Nanogel viscosity (Y2), and gel diffusion in-vitro (Y3). Besides, pH, homogeneity, spreadability, viscosity, extrudability and drug content tests characterized the formulation. The nanoparticles were characterized through particle size, polydispersity index (PDI), zeta potential, encapsulation efficiency and Percentage yield. The resulting shave found F5was best formulation among all batches and has transformed into topical Nanogel. In order to find the compositions of the optimised formulation, the statistical validity of the polynomials was established. Further response surface plots were drawn. Therefore, it can be inferred that the design shows the role of the derived polynomial equation and contour plots in predicting the values of dependent variables for preparation and optimization of Nanogel formulation. It was concluded that the GTE-loaded Nanogel was stable and could be used for the delivery of topical drugs with promising potential.