Abstract

*M. Murali Krishna, R. Venkateswarlu, A. Harsha Vardhan, A. Mahesh Reddy, Ch. Geetha, P. Suseela and YVS. Rajeswari

ABSTRACT

A simple reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous determination of tinidazole and diloxanide furoate. The separation was carried out using a mobile phase consisting of acetonitrile and 0.2 M potassium dihydrogen phosphate (pH 5) in the ratio 45:55% v/v. The column used was Hypersil OSD C18 (250 x 4.6 mm, 5 ? particle size) with a fl ow rate of 1 ml/min and UV detection at 278 nm. The described g/ml for the assay of diloxanide furoate and?g/ml and 25-150 ?method was linear over a concentration range of 30-180 tinidazole, respectively. The mean recovery was found to be 100-101% for tinidazole and 97-103% for diloxanide furoate when determined at three different levels.