Abstract

Khyati Chandra, Raison Thomas, Rucha Shah* and Dhoom Singh Mehta

ABSTRACT

Objectives: The present study was carried out with the aim of evaluating the antibacterial activity of Plectranthus Amboinicus extract by assessing its Minimum inhibitory concentrations (MIC) and Minimum bactericidal concentration (MBC) on the most common periodontal pathogens, Porphyromonas gingivalis(Pg), Prevotella intermedia(Pt), Fusobacteria nucleatum(Fn) and Aggregatibacter actinomycetemcomitans(Aa) followed by the determination of its invivo efficacy as topical gel in the management of chronic gingivitis. Methods: The assessment of Minimum inhibitory concentrations (MIC) and Minimum bactericidal concentration (MBC) of Plectranthus Amboinicus on Pg, Pt, Fn and Aa was carried out using serial dilution method. Subsequently the clinical effect of application of P Amboinicus gel as topical gel application in 24 (10 men and14 women) chronic gingivitis patients was assessed. The test group patients were advised to use P Amboinicus Gel after scaling and these patients were examined after 21 days. The control group patients were not given any gel application and also were examined after 21 days. Results: The results indicated that P. Amboinicus extract was effective at concentration of 50?g/ml against four periodontopathogenic bacteria. From the MBC Values it was deduced that at there was no growth of Pg,Pi,Fn at the MIC of 50 ?g/ml after inoculation into the culture media. There was minimal growth of Aa seen at the same concentration of 50 ?g/ml. The results of the clinical trial indicated that from the baseline till the completion of the study at 21 days, there was statistically significant decrease recorded in plaque index, gingival index in the test group when compared to the control group (p=0.000). No patients showed any adverse reaction or reported any discomfort with the P Amboinicus gel. Conclusion: Topical application of Plectranthus Amboinicus gel played an effective role in controlling chronic gingivitis