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World Journal of Pharmaceutical
and Medical Research

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
ISSN 2455-3301
IMPACT FACTOR: 5.922

ICV : 78.6

Abstract

METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF TERBINAFINE & ITRACONAZOLE BY RP-HPLC METHOD

Yalla Chandana*, Dr. Devanaboyina Narendra, P. Venkata Kishore and Gadi Vijaya Lakshmi

ABSTRACT

A simple, Accurate, precise method was developed for the simultaneous estimation of the Terbinafine and Itraconazole in tablet dosage form. Chromatogram was run through Agilent C18 150 x 4.6mm, 5.0?. Mobile phase containing Buffer 0.01N KH2PO4 (4.8pH) : Acetonitrile taken in the ratio 60:40v/v was pumped through column at a flow rate of 0,8 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 270nm. Retention time of Terbinafine and Itraconazolewere found to be 2.340 min and 2.940min. %RSD of the Terbinafine and Itraconazole were and found to be 1.2 and 1.2 respectively. %Recovery was obtained as 99.56% and 100.16% for Terbinafine and Itraconazole respectively. LOD, LOQ values obtained from regression equations of Terbinafine and Itraconazole were 0.77, 2.34 and 1.33, 3.42 respectively. Regression equation of Terbinafine is y = 1316.x + 560.8 and y = 2912x + 3286 of Itraconazole. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

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