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World Journal of Pharmaceutical
and Medical Research

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
ISSN 2455-3301
IMPACT FACTOR: 5.922

ICV : 78.6

Abstract

METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF SEGESTERONE & ETINYL ESTRADIOL BY RP-HPLC METHOD

Butt Khadheeja*, Dr. Devanaboyina Narendra and Gadi Vijaya Lakshmi

ABSTRACT

A simple, Accurate, precise method was developed for the simultaneous estimation of the Segesterone and Ethinyl Estradiol in syrup dosage form. Chromatogram was run through AgilentC18 150 x 4.6 mm, 5?. Mobile phase containing Buffer 0.01N KH2PO4 (5ph): Acetonitrile taken in the ratio 65:35 was pumped through column at a flow rate of 1.0ml/min. Buffer used in this method was 0.01N KH2PO4. Temperature was maintained at 30°C. Optimized wavelength selected was 260nm. A simple, Accurate, precise method was developed for the simultaneous estimation of the Segesterone and Ethinyl Estradiol in syrup dosage form. Retention time of Segesterone and Ethinyl Estradiol were found to be 2.302 min and 3.324min. %RSD of the Segesterone and Ethinyl Estradiolwere and found to be 0.6 and 1.0 respectively. %Recovery was obtained as 100.33% and 99.45% for Segesterone and Ethinyl Estradiol respectively. LOD, LOQ values obtained from regression equations of Segesterone and Ethinyl Estradiolwere 0.91, 2.75and 0.04, 0.11 respectively. Regression equation of Ethinyl Estradiol is y = 14306x + 2432.1. And y = 7317.8x + 2228.5. of Segesterone. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

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