BEHAVIORAL EVIDENCE OF ANTIDEPRESSANT-LIKE ACTIVITY OF CASSIA SIEBERIANA EXTRACT IN MICE
Bakou Niangoran François*, B. A. Abdoulaye, Diabate D. and Atayi E.
Objective: The present study was designed to evaluate the acute and chronic behavioral and antidepressant effects of hydro alcohol extract of stem bark of Cassia sieberiana in standardized mice models of depression. Materials and Methods: hydro ethanolic extract of stem bark from Cassia sieberiana were prepared, and phytoconstituents were determined using appropriate chemical analytical methods. Animals were divided into five groups (n=5/group): The control group received vehicle (saline water 0,1ml/mouse). Amitriptyline (20mg/kg b.w., IP) was used as the positive control or standard group while the treated mice received CS (100, 200, and 400mg/kg body weight i.p). In the acute treatment study, a single dose was administered 30 min prior to testing. For the chronic treatment study, a single dose was administered daily for 14 days. In the chronic dose study, the behaviors of all groups were assessed for antidepressant activity 30 min after the last treatment dose on the 14th day. Different standardized depression models were used for behavioral tests to evaluate the antidepressant activity, such as forced swim test (FST), and tail suspension test (TST) test. Results: the preliminary pharmacological screening with acute dosing exhibited the antidepressant activity of CS, but its antidepressant activity was more enhanced after repeated dosing. In comparison with the acute studies, chronic dose studies displayed a significant antidepressant manifestation in the behavioral patterns when compared to the vehicle controls. Conclusion: The results obtained in this work suggest that the hydro ethanolic extract of stem bark from Cassia sieberiana may possess an antidepressant activity.[Full Text Article]