Abstract

Gadi Vijaya Lakshmi* and Dr. Devanaboyina Narendra

ABSTRACT

A simple, Accurate, precise method was developed for the simultaneous estimation of the Dapagliflozin and Saxagliptin in Tablet dosage form. Chromatogram was run through Std BDS 150 x 4.6 mm, 5?. Mobile phase containing Buffer Perchloric acid: Acetonitrile taken in the ratio 50:50 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA. Temperature was maintained at 30°C. Optimized wavelength selected was 220 nm. Retention time of Dapagliflozin and Saxagliptin were found to be 2.266min and 2.805min. %RSD of the Dapagliflozin and Saxagliptin were and found to be 0.9 and 0.6 respectively. %Recovery was obtained as 99.72% and 99.60% for Dapagliflozin and Saxagliptin respectively. LOD, LOQ values obtained from regression equations of Dapagliflozin and Saxagliptin were 0.12, 0.36 and 0.02, 0.06 respectively. Regression equation of Dapagliflozin is y = 20173x + 18271, and y = 4124x + 2572 of Saxagliptin. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.