Abstract

Sangaru Madhava Rao*, Dr. Devanaboyina Narendra, Gadi Vijaya Lakshmi and P. Venkata Kishore

ABSTRACT

A simple, Accurate, precise method was developed for the simultaneous estimation of the Betamethasone, Gentamicin and Miconazole in liquid dosage form. Chromatogram was run through Phenomenex C18 150 4.6 mm, 5?. Mobile phase containing Buffer and Acetonitrile in the ratio of 35:65 A was pumped through column at a flow rate of 1ml/min. Buffer used in this method was Water at Temperature was maintained at 30°C. Optimized wavelength for Betamethasone, Gentamicin and Miconazole was 240 nm. Retention time of Betamethasone, Gentamicin and Miconazole were found to be 2.177min, 3.321 min and 2.634 min %RSD of system precision for Betamethasone, Gentamicin and Miconazole. were and found to be 0.8, 0.8 and 0.7 respectively. %RSD of method precision for Betamethasone, Gentamicin and Miconazole. were and found to be 0.4, 0.5 and 0.3respectively. % recovery was Obtained as 99.56%,99.37%,99.16% for Betamethasone, Gentamicin and Miconazole. Respectively. LOD values are obtained from regression equations of Betamethasone, Gentamicin and Miconazole were 0.09ppm, 0.04ppm, 1.96ppm. LOQ Values 0.28ppm and 0.12ppm, 5.93ppm respectively. Regression equation of Betamethasone was y =3415.x + 485.6, Gentamicin was y = 5211.x +876.6 and of Miconazole was y =4497 .x + 7864.