Abstract

Ponnamalla Jasmine Carey*, Dr. Devanaboyina Narendra and Gadi Vijaya Lakshmi

ABSTRACT

A simple, Accurate, precise method was developed for the simultaneous estimation of the abacavir, lamivudine and dolutegravir tablet dosage form. Chromatogram was run through STD Agilent C18 150 x 4.6 mm, 5?. Mobile phase containing Water: Acetonitrile taken in the ratio 60:40 was pumped through column at a flow rate of 0.8 ml/min. Buffer used in this method was milli-Q Water. Temperature was maintained at 30°C. Optimized wavelength selected was 284 nm. Retention time of Abacavir/Lamivudine/ Dolutegravir were found to be 2.233 min and 2.700,3.426. %RSD of the Abacavir/Lamivudine/ Dolutegravir were and found to be 0.4,0.5 and 0.3 respectively. %Recovery was obtained as 99.89%, 100.0.4%and 100.35% for abacavir/Lamivudine/ dolutegravir respectively. LOD, LOQ values obtained from regression equations of Abacavir/Lamivudine/ dolutegravir were 0.04, 0.11,0.08 and 0.13, 0.33,0.24respectively. Regression equation of Abacavir is y = 37926x + 8085.1, y = 38697x + 13271 of Lamivudine and dolutegravir is y= 4326.9x+194.59. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular quality control test in Industries.