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World Journal of Pharmaceutical
and Medical Research

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
ISSN 2455-3301
IMPACT FACTOR: 5.922

ICV : 78.6

Abstract

COMPARISON PLATELETS COUNT BY AUTOMATED AND MANUAL HAEMOCYTOMRTER METHODS

Amal Elhassade*, Mahbuba Hassan, Hajir Geryat, Boshra Elkelani and Salma Aljbali

ABSTRACT

Background: Manual platelet counting in the Neubauer chamber, by means of a phase-contrast microscope, has been recommended as the reference method for assessing the platelet number by the International Committee for Standardization in Hematology (ICSH -1984). platelet counts as a reference for the calibration of hematology analyzers has been a continuing problem due to varying results. Methods: Fifty-Five blood samples in EDTA-anticoagulant vacutainer tubes were obtained and randomly divided into 3 group; Group A-normal control (n =34), Group b thrombocytopenia (n = 14) and Group C high platelets count thrombocytosis (n = 6). Each blood sample were analyzed for platelet count by manual microscopy, and automated hematology analyzer. The agreement between the two methodologies were assessed using the paired t-test and correlation coefficient analyses. Results: The coefficient of variation values significantly differed in Group A and were high for Automated cell counter. High positive correlation was also observed between Automated cell counter values and manual method (r=0.935, p=0.001) in group b was not significantly correlated. Conclusion: Based on the results observed in thrombocytopenia, manual platelet count using by haemocytometry may be promising if platelets are very low and giant platelets.

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