Abstract

Nilesh Patel, Dr. Janmejay Patel*, Achal Patel, Dr. Ankitkumar M. Paneliya*

ABSTRACT

Introduction: World Health Organization estimated that 80% of the world's population still depend on traditional medicines for their health care despite the availability of advanced medicinal systems. The toxicological evaluation of herbal formulation is necessary as it is in use for wide variety of clinical applications. Aim: To evaluate Rhumavin Tablet for its acute oral toxicity on Swiss albino mice and efficacy with anti-oxidant properties on arthritic rats. Method: The study protocol was approved by IAEC (SKPCPER/IAEC/2016-02/05) as per the CPCSEA. The acute oral toxicity was assessed by following OECD guideline AOT-425 to know single dose (2000 mg/kg) toxicity of test drug. Animals were periodically observed individually for 14 days for any clinical signs of toxicity or mortality after administration of test drug. Bovine serum denaturation assay (In-vitro) was done to examine effectiveness of test drug on arthritis. FCA induced arthritis animal model (In-vivo) was adopted to assess effectiveness of test drugin arthritis. FCA was challenged to sub plantar region after treatment in Rats and various parameter such as paw volume, arthritic index, hematological and radiological parameterswere measured. Different assay analysis (total protein, SOD activity, Catalase activity, Lipid peroxidation) were done to establish its anti-oxidant properties. Results: There were no any physical as well as behavioral changes and mortality observed in any animal during study period. Body weight of all animals did not reveal any significant change as compared to vehicle control group. Rhumavin Tablet has significantly decreased paw volume, arthritic index, hematological parameters compared to disease control group. The significant increase in SOD and calalase level was observed in test drug treated group. Conclusion: The No-Observed-Adverse-Effect-Level (NOAEL) of Rhumavin Tablet is 2000 mg/kg. The obtained results advocate anti- inflammatory, anti-arthritic and antioxidant effect of Rhumavin Tablet.