Abstract

Vardhanapu Mary Bharathi*, Dr. Devanaboyina Narendra, Gadi Vijaya Lakshmi, Yendamuri Narayudu, Penumantra Venkata Kishore

ABSTRACT

A simple, Accurate, precise method was developed for the simultaneous estimation of the L-methyl folate and Escitalopram in Tablet dosage form. Chromatogram was run through Waters C18 150 x 4.6 mm, 5?. Mobile phase containing Buffer 0.01N KH2PO4 (3.45pH): Acetonitrile taken in the ratio 50:50 was pumped through column at a flow rate of 1.0 ml/min. Buffer used in this method was 0.01N KH2PO4. Temperature was maintained at 30°C. Optimized wavelength selected was 212 nm. Retention time of L-methyl folate and Escitalopram were found to be 2.263min and 3.079min. %RSD of the L-methyl folate and Escitalopram were and found to be 0.2 and 0.5 respectively. %Recovery was obtained as 99.79% and 100.54% for L-methyl folate and Escitalopram respectively. LOD, LOQ values obtained from regression equations of L-methyl folate and Escitalopram were 0.24, 0.29 and 0.72, 0.87 respectively. Regression equation of L-methyl folate is y = 22979x + 8347. and y = 31569x + 3873. of Escitalopram. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.