Abstract

Dr. Vishva Bhuva*, Dr. Komal Hirani, Payal Panchal and Amit Patel

ABSTRACT

Introduction: Ayurvedic texts describe many formulations for different ailments. Abha capsule is reputed for treating bone fracture and osteoporosis. These formulations have been considered complementary medicine or alternative to conventional medicines across the globe. These complex polyherbal formulations need science based approach toward manufacturing process and chemical standardization. Aim: Study was to find out a simple, accurate and sensitive HPTLC method for the detection and quantification of marker molecules of raw material and comparison with finished product for standardization. Material: A simple and reproducible high performance thin layer chromatography method for the determination of active component of Ayurvedic product i.e. Abha capsule has been performed for standardize formulation. Result: The key ingredients of Abha capsule are Abha guggulu, Kaishore guggulu, Lakshadi guggulu and Hadjod. Guggulu is common ingredient of all three preparations and Hadjod is the key ingredient of Formulation. Investigation shows Gallic acid, Piperine and gingerol is present in methanolic extract of Abha guggulu, Kaishore guggulu and Abha capsule. Although Lakshadi guggulu and Abha capsule contains Withanolide. HPTLC chromatogram of Abha guggulu, Kaishore guggulu, Lakshadi guggulu shows three similar peaks at Rf 0.21,0.43,0.54,0.74 which is also seen in Abha capsule which indicate presence of guggulu in Abha capsule. Hadjod extract analysis shows peaks at Rf 0.26, 0.41, 0.58, & 0.83, which also appear in extract of Abha capsule. Conclusion: The quality of formulation proves by presence Hadjod, Guggulu and standards like Gallic acid, Piperine, Gingerol and Withanolide in Abha capsule.