Abstract

Dr. Bhupendra Kumar Mandawat*

ABSTRACT

Background: The spread of Extended Spectrum Beta-Lactamases (ESBLs) producing bacteria has become strikingly rapid worldwide, indicating that continuous monitoring systems and effective infection control measures are absolutely required. The therapeutic options for the infections which are caused by these organisms have also become increasingly limited. ESBLs producing strains have emerged as a significant challenge to counter with present antibiotics. Objectives: The aims of this study were to detect prevalence of ESBL production among Klebsiella pneumoniae and to detect their antibiotic susceptibility pattern at Govt. Medical College, Kota. This study demonstrate the importance of regular review of empirical antibiotic therapy in view of the evolving resistance of ESBL producing Klebsiella pneumoniae to commonly used antimicrobial agents. Methodology: A total of 101 consecutive, nonrepetitive Klebsiella pneumoniae isolates were studied. These isolates were confirmed for ESBL production by the double disc synergy test (DDST) and the phenotypic confirmatory disc diffusion test (PCDDT) and Twenty-five randomly selected isolates were further confirmed by the E-test ESBL strip randomly. Out of 101 Klebsiella pneumoniae isolate, 59(58.42%) were ESBL producers and 42(41.58%) were Non ESBL producers. Out of the 59 isolates which were tested,42(71.19%)were found to be ESBL producers by PCDDT,35(59.32%)were found to be ESBL producers by DDST and 18(72%), were found to be ESBL producers by the E-test ESBL strip which showed a highly significant correlation with PCDDT. Conclusion: As results showed that there was a high prevalence of ESBL production in our setup so, it is essential to report the ESBL production along with the routine sensitivity reports, which will help the clinician in selection of proper antibiotics.