Abstract

A. Manoj, B. Vishnu Bhat, C. Venkatesh and Z. Bobby

ABSTRACT

Cytokinesis-Block Micronucleus (CBMN) Assay is a promising method to detect Double strand breaks of DNA and Chromosome Instablity in somatic cells. This assay has been extensively used to evaluate the presence and the extent of DNA and chromosome damage in human populations exposed to Free radicals scavengers and genotoxic agents in various occupational settings, in the environment, or as a consequence of lifestyles. The technique has been done in dividing cell in invitro culture by blocking karyokinesis and cytokinesis with Cytochalasisn-B inorder to check whether the double strand break of DNA in the form chromatid breaks or mal-segregation of chromosome. Mutations of chromosome includes lagging of whole chromosomes, acentric chromosome fragments which is attributed by mutations of kinetochore proteins, centromeres and spindle apparatus leads to Micronucleus formation. Miconuclei are morphologically identical to nucleus but smaller with similar staining intensity as nucleus within an intact cytoplasm. The micronucleus index (MNi) for each sample was analysed by counting 1000 binucleated cells by manual method based on the scoring criteria framed by International collaborative project on micronucleus frequency HUMN project.