Abstract

Laxman S. Vijapur, Jayadev N. Hiremath, Nikhil N. Bonageri* and Anitha R. Desai

ABSTRACT

Silver nanoparticles were successfully synthesized using silver nitrate and Murraya koenigii leaves extract by varying the concentration of hydroalcoholic extract. The Murraya koenigii leaves extract was containing carbohydrates, flavonoids, phenolic and alkaloids as phyto-constituents. Formation of Silver nanoparticle with phytochemiclas was confirmed by UV-Vis spectra by observing peak absorption at 411.0 nm & 423.5 nm. FT-IR spectroscopy showed the capping of silver was due to phenols and alkaloids. Dynamic light scattering of the prepared formulations revealed all the formulations were within nano range, SN1 showed average particle diameter 170.7 nm with zeta potential -22.51 mV, SN2 showed particle diameter was 67.5 nm with zeta potential -18.14 mV, and SN3 showed average particle diameter of 64.1 nm with zeta potential -12.56 mV and SN4 formulation showed the average particle diameter of 58.4 nm with zeta potential -11.89 mV, SN5 formulation showed the average particle diameter of 46.9 nm with zeta potential -8.92 Mv, out of the above formulation SN5 was having least average particle diameter. Atomic force microscope showed that the particles were having smooth surface in the range from 29.4 nm to 52.0 nm for SN5 formulation. X-ray diffraction of the formulation showed the structural information of silver nanoparticle which were FCC in shape. Haemolytic studies revealed that the there was negligible haemolysis of erythrocytes when nanoparticles were used in the concentration of 100?gm/ml thus prepared formulation were bio-compatible. MTT assay of optimized formulation SN5 compared with pure hydroalcoholic extract showed a dose dependent anticancer activity against MDA-MB-231 cell lines. The IC50 for hydroalcoholic extract was 2.40 mg\ml whereas for synthesized AgNps the IC50 was 6.522 ?g\ml only.