TO ASSESS THE ROLE OF VARIOUS IMMUNOHISTOCHEMICAL MARKERS IN CLASSIFICATION OF LUNG CARCINOMA ON ENDOBRONCHIAL BIOPSIES
Namita Bhutani, Nisha Marwah, Dhruv Chaudhary, Pradeep Kajal* and Rajeev Sen
ABSTRACT
Background: Lung cancer is the most frequently diagnosed cancer and leading cause among cancer mortality worldwide. An accurate classification is difficult in small biopsy specimens due to a variety of reasons. Therefore, there is an increasing need for additional diagnostic techniques such as immunohistochemistry. Methods: This study was conducted in Pt. B D Sharma, PGIMS, Rohtak. Endobronchial biopsies of One hundred and sixty patients were subjected to routine H & E and IHC staining. Results: The patients were in age group of 25-75 years with a mean of 55.67 years with M: F ratio of 6.61:1. NSCLC constituted the major type, contributing to 83.1% of cases. Amongst the NSCLC, poorly differentiated subtype topped the list, with 53.7% cases. p63 was highly sensitive (98.13%) and specific (100%) for squamous cell carcinoma. Amongst, TTF-1 and napsin-A, the later had higher sensitivity (96.15%) as compared to TTF-1 (92.30%) for diagnosing adenocarcinoma. Conclusion: CK and p63 served as highly sensitive markers for diagnosis of squamous cell carcinoma and TTF-1 and napsin A for adenocarcinoma, forming an important diagnostic algorithm for subtyping of poorly differentiated NSCLC on small biopsies.
[Full Text Article] [Download Certificate]