Abstract

*A. Manoj and Annamma Paul

ABSTRACT

This research work was conducted to study the microscopic Anatomy of Liver inorder to identify the normal histological features of liver in low and high magnifications with aim of establishing a basic understanding, which will help recognise pattern of injury in liver diseases. Liver tissue was preserved in formalin and embedded in paraffin wax to obtain translucent sections. Paraffiin sections were stained with Haemotoxylin and Eosin which had observed under 10X magnification delineated hepatic lobule demarcation had been evidencing by Glisson capsule which condenses at portal triad at six angles with single central vein at centre. Under 40X magnification hexagonal lobule had polyhedral hepatocytes were radially arranged with two cell thickness encloses sinusoids, connective tissue stroma, the portal triad contains branches of Portal vein, hepatic artery and bile ductile. Based on bile secretion three adjacent hepatic lobule delivered into an axial hepatic ductile of triangular portal lobule formed from imaginary line connecting three central veins. Diamond shaped parenchymal acinus provides the best association between the perfusion of blood, metabolic activity and liver derangement made by imaginary line connecting two central veins and two portal triad. Alteration on the normal histological architecture being suggestive of liver disorders which can be identified in histopathological sections and to be reflected in the functions of liver.