Abstract

Chadalawada Madhuri* and D. Dhachinamoorthi

ABSTRACT

The main objective of the present study is estimate the % adsorption of recombinant protein antigen on the reduced aluminium phosphate adjuvant particles. Bovine serum albumin was used as a working standard for estimation of protein by lowry method. Bovine serum albumin was studied using with the concentration from 20, 40, 60,80and 100 ?g/mL. Different size containing aluminium phosphate adjuvant particles are prepared by using homogenization, sonication, freezed and thawed methods. Homogenization study is conducted at 10,000 rpm by different time intervals i.e. 15, 30, 60, and 90 minutes. Sonication study was conducted by using elma sonicator at different time intervals i.e. 60 and 180 minutes. During sonication the alumnium phosphate particle size is reduced from 2.20?m to 1.82?m. Freezed at -20C and thawed at room temperature. Different size containing aluminium phosphate particles are estimated by using xylenol orange as an indicator. From the above methods the homogenization at 10,000 rpm at 90 minutes (Batch-8) has less particle size hence the rate of adsorption of recombinant protein antigen is also high. Sonicated aluminium particles size is greater than homogenized particles hence the rate of adsorption is less than homogenized particles. It was conclude that the rate of shearing and time plays a major role for reduced particle size during homogenization and shows better adsorption.