Abstract

H. D. Bandhavya* and K. C. Chaluvaraj

ABSTRACT

Aim: To simultaneously estimate Atorvastatin and Gemfibrozil using UV-Spectroscopic method. Methodology: Different simultaneous estimation techniques were used for estimation of Atorvastatin and Gemfibrozil using UV spectroscopy. The methods used were simultaneous equation method, area under curve (AUC) method, Q-analysis or absorbance ratio method. The developed method was validated by following ICH guidelines. Results: The absorption spectra was recorded in wavelength region of 200-400nm. Linearity of ATR and GEM was found 2-14μg/ml and 30-90μg/ml respectively. In this work the ATR and GEM were quantified by using three different quantitative methods of UV spectroscopy. The proposed methods confirm the suitability for the estimation of physical mixture of pure drugs as well as for pharmaceutical formulation in combination. The coefficient of correlation for ATR at 223 nm and GEM at 272 nm by all methods was found to be within the range of 0.9738-0.9995 and 0.9798-0.9995 respectively. Both drugs showed good regression values at their respective wavelengths in the all methods. The suggested method's accuracy was tested by estimating pure drug and pharmaceutical formulation, and the results were computed, with no interference from other typical excipients found in pharmaceutical formulations. The presented approach was found to be simple, sensitive, accurate, precise, and cost-effective, and it may be used to determine ATR and GEM individually or as a physical mixture for regular analysis.